Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: p53 , REA1132 , 50 , 130-119-502 , PE , Miltenyi Biotec.

Techniques: Imaging

Journal: Antioxidants

Article Title: Saposhnikovia divaricata Inhibits Inflammation, Oxidative Stress, and Ferroptosis to Alleviate DSS-Induced Ulcerative Colitis

doi: 10.3390/antiox15020258

Figure Lengend Snippet: Network pharmacology analysis identifies p53 as a core ferroptosis-related target of FF in UC. ( A ) Venn diagram illustrating the intersection of FF compound targets with ferroptosis- and UC-related targets. ( B ) Protein–protein interaction (PPI) network of the common targets. Node size and color intensity represent the degree of connectivity, with TP53 (p53) identified as the core target. ( C ) Compound-target-pathway network diagram. The inner pink nodes represent the 38 intersecting targets linking FF, UC, and ferroptosis. ( D ) Gene Ontology (GO) enrichment analysis of the common targets, categorized into Biological Process (BP, red), Cellular Component (CC, green), and Molecular Function (MF, blue). ( E ) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis.

Article Snippet: The p53 antibody was obtained from Boster Biological Technology Co., Ltd. (Pleasanton, CA, USA, Item #BM0101).

Techniques:

Journal: Antioxidants

Article Title: Saposhnikovia divaricata Inhibits Inflammation, Oxidative Stress, and Ferroptosis to Alleviate DSS-Induced Ulcerative Colitis

doi: 10.3390/antiox15020258

Figure Lengend Snippet: FF modulates the expression of ferroptosis-related proteins in colon tissue via the p53 pathway. ( A ) Representative immunohistochemical (IHC) images of p53, SLC7A11, and GPX4 expression in colon sections (scale bar = 50 μm). ( B – D ) Quantitative analysis of the relative protein expression levels of p53 (B), SLC7A11 (C), and GPX4 (D). Data are presented as the mean ± SD ( n = 3 independent experiments). ### p < 0.001 versus the control (CON) group; * p < 0.05, ** p < 0.01, *** p < 0.001 versus the DSS model group.

Article Snippet: The p53 antibody was obtained from Boster Biological Technology Co., Ltd. (Pleasanton, CA, USA, Item #BM0101).

Techniques: Expressing, Immunohistochemical staining, Control

Journal: Journal of Clinical Laboratory Analysis

Article Title: High‐dimensional single‐cell proteomics analysis reveals the landscape of immune cells and stem‐like cells in renal tumors

doi: 10.1002/jcla.23155

Figure Lengend Snippet: Purified antibodies about the stem‐like cell‐centric panel

Article Snippet: p53 , 143Nd , 7F5 , Fluidigm , 3143018A.

Techniques: Purification

Journal: Cancer

Article Title: A highly sensitive particle agglutination assay for the detection of P53 autoantibodies in patients with lung cancer.

doi: 10.1002/cncr.23057

Figure Lengend Snippet: FIGURE 1. Reactivity of superparamagnetic particles coated with p53 (amino

Article Snippet: Superparamagnetic particles with carboxylic acid groups (Dynabeads M-270; Invitrogen, Krefeld, Germany) were coated with p53 peptides from the NH2 terminus (peptide 1; amino acid [AA]15-AA29), the COOH terminus (peptide 2; AA314-AA326), the wildtype p53 protein (Dianova), and a monoclonal antibody to p53 (mouse antihuman p53 clone DO-2; Serotec, Dusseldorf, Germany).

Techniques:

Journal: Cancer

Article Title: A highly sensitive particle agglutination assay for the detection of P53 autoantibodies in patients with lung cancer.

doi: 10.1002/cncr.23057

Figure Lengend Snippet: FIGURE 2. Detection of p53 autoantibodies (Abs) in randomly selected

Article Snippet: Superparamagnetic particles with carboxylic acid groups (Dynabeads M-270; Invitrogen, Krefeld, Germany) were coated with p53 peptides from the NH2 terminus (peptide 1; amino acid [AA]15-AA29), the COOH terminus (peptide 2; AA314-AA326), the wildtype p53 protein (Dianova), and a monoclonal antibody to p53 (mouse antihuman p53 clone DO-2; Serotec, Dusseldorf, Germany).

Techniques:

Journal: bioRxiv

Article Title: Senescence-inhibitory Δ133p53α counteracts accelerated ageing and mortality

doi: 10.64898/2025.12.31.697195

Figure Lengend Snippet: Western blot analyses of lamin A/C and progerin, full-length p53, Δ133p53α, and p21 Waf1/Cip1 were performed in four 15-week-old Δ133p53α-expressing Group-1 mice ( CAG-133 Tam/+ ; Cre Tg/+ ; Lmna G609G/+ ), along with four each of age-matched, non-expressing control Group-2 ( CAG-133 LSL/+ ; Cre Tg/+ ; Lmna G609G/+ ) and Group-4 mice ( CAG-133 +/+ ; Cre +/+ ; Lmna G609G/+ ), as well as two age-matched wild-type mice ( CAG-133 +/+ ; Cre +/+ ; Lmna +/+ ). Results from skin ( a ), skeletal muscle ( b ), kidney ( c ), spleen ( d ), and lung ( e ) are presented. An inter-blot control (liver from a Group-1 mouse) was included in all blots. F, female; M, male. GAPDH was a loading control and used for normalization of p21 Waf1/Cip1 , progerin, and full-length p53 expression levels. Quantitative data summaries of p21 Waf1/Cip1 , progerin, and full-length p53 in Group-1, -2 and -4 mice are shown as relative values to Group-2 mice (mean ± s.d. from n = 4; open circles indicate two females, and closed circles indicate two males). P values were determined by Welch’s t -test. Two wild-type mice were used only as references and not for statistical comparisons.

Article Snippet: Primary antibodies used were as follows: anti-p53 antibodies SAPU (sheep polyclonal) and DO-11 (mouse monoclonal; Bio-Rad, MCA1704) ; anti-p21 Waf1/Cip1 (mouse monoclonal; Santa Cruz Biotechnology, sc-6246, clone F-5); anti-lamin A/C (mouse monoclonal; Santa Cruz Biotechnology, sc-376248); anti-GAPDH (mouse monoclonal; Santa Cruz Biotechnology, sc-166574); and anti-β-actin (mouse monoclonal; Thermo Fisher Scientific, MA1-91399).

Techniques: Western Blot, Expressing, Control

Journal: bioRxiv

Article Title: Senescence-inhibitory Δ133p53α counteracts accelerated ageing and mortality

doi: 10.64898/2025.12.31.697195

Figure Lengend Snippet: a,b , Top Hallmark pathways identified by Gene set enrichment analysis (GSEA). The bulk RNA-seq data were obtained from the heart ( a ) and kidney ( b ) in 9-10-month-old Group-1 and Group-3 mice (n = 5 each). Pathways are ranked by normalized enrichment score. False discovery rate < 0.10. c-g, Enrichment plots illustrate significant downregulation of the p53 pathway ( c,d ) and upregulation of the oxidative phosphorylation ( e,f ) in both heart and kidney, as well as upregulation of the glycolysis in the kidney ( g ). Enrichment plots depict running enrichment scores (ES) and the distribution of genes within each Hallmark gene set. All leading edge genes in each pathway are listed in Extended Data Table 2. h,i, qRT-PCR assays of mRNA expression of genes in the oxidative phosphorylation pathway (Ndufs6, Ndufc2, Uqcrq, Hsd17b10, and Gpx4) and an antioxidant gene Prdx1 in the heart ( h ) and kidney ( i ) of 9-10-month-old Group-1 and Group-3 mice (mean ± s.d. from n = 5, each with technical triplicate; open circles, females; closed circles, males). P values were calculated by Welch’s t -test.

Article Snippet: Primary antibodies used were as follows: anti-p53 antibodies SAPU (sheep polyclonal) and DO-11 (mouse monoclonal; Bio-Rad, MCA1704) ; anti-p21 Waf1/Cip1 (mouse monoclonal; Santa Cruz Biotechnology, sc-6246, clone F-5); anti-lamin A/C (mouse monoclonal; Santa Cruz Biotechnology, sc-376248); anti-GAPDH (mouse monoclonal; Santa Cruz Biotechnology, sc-166574); and anti-β-actin (mouse monoclonal; Thermo Fisher Scientific, MA1-91399).

Techniques: RNA Sequencing, Phospho-proteomics, Quantitative RT-PCR, Expressing

Journal: International Journal of Cancer

Article Title: Bcl-2 with loss of apoptosis allows accumulation of genetic alterations: A pathway to metastatic progression in human breast cancer

doi: 10.1002/(sici)1097-0215(20000320)89:2<142::aid-ijc7>3.0.co;2-b

Figure Lengend Snippet: FIGURE 1 – Percentage of tumors with Bcl-2 over-expression according to the number of accumulated oncogene alterations (c-Myc, EGFR and c-erbB-2) and p53 status (mutated vs. non-mutated tumors).

Article Snippet: Bcl-2, c-Myc, c-erbB-2, p53, EGFR and hormone receptor (PR, ER) expression Expression of these genes was assessed by immuno-histochemistry (IHC) using appropriate antibodies: the monoclonal antibody (MAb) anti-Bcl-2 124 (ICI, Cambridge Research Biochemicals, Northwich, UK) at 1:200 dilution; MAb to p53, clone BP-53-12-1 (Biogenex, San Ramon, CA); MAb Ab-6, clone DO-1 (Oncogene Science, Uniondale, NY); MAb Ab-1, clone 9E10 (Oncogene Science), which reacts with human c-Myc at 1:100 dilution; MAb anti-C-erbB-2, clone CB11 (Biogenex) at 1:80 dilution; MAb anti-EGFR, clone E30 (Merck, Darmstadt, Germany) at 1:15 dilution.

Techniques: Over Expression

Journal: International Journal of Cancer

Article Title: Bcl-2 with loss of apoptosis allows accumulation of genetic alterations: A pathway to metastatic progression in human breast cancer

doi: 10.1002/(sici)1097-0215(20000320)89:2<142::aid-ijc7>3.0.co;2-b

Figure Lengend Snippet: FIGURE 2 – Percentage of tumors with p53 over-expression according to the number of accumulated oncogene alterations (c-Myc, EGFR and c-erbB-2) and histological grade (I/II or III).

Article Snippet: Bcl-2, c-Myc, c-erbB-2, p53, EGFR and hormone receptor (PR, ER) expression Expression of these genes was assessed by immuno-histochemistry (IHC) using appropriate antibodies: the monoclonal antibody (MAb) anti-Bcl-2 124 (ICI, Cambridge Research Biochemicals, Northwich, UK) at 1:200 dilution; MAb to p53, clone BP-53-12-1 (Biogenex, San Ramon, CA); MAb Ab-6, clone DO-1 (Oncogene Science, Uniondale, NY); MAb Ab-1, clone 9E10 (Oncogene Science), which reacts with human c-Myc at 1:100 dilution; MAb anti-C-erbB-2, clone CB11 (Biogenex) at 1:80 dilution; MAb anti-EGFR, clone E30 (Merck, Darmstadt, Germany) at 1:15 dilution.

Techniques: Over Expression

Journal: BMC Dermatology

Article Title: SIRT1 activation mediates heat-induced survival of UVB damaged Keratinocytes

doi: 10.1186/s12895-017-0060-y

Figure Lengend Snippet: Exposure to repeated UVB plus heat significantly decreased acetylated p53 levels in keratinocytes. Representative immunohistochemical staining of ex vivo skin either untreated, or exposed to heat, UVB or UVB plus heat, for nuclear DNA (DAPI, blue ), CPD ( red ) and ( a ) phosphorylated SIRT1 (SIRT1-p), ( b ) caspase (casp-3), ( c ) total p53 or ( d ) acetylated p53 (p53-a382). Inset images are an enlarged view of cells positive for CPD and SIRT1 ( red arrows ), CPD and Casp-3 ( orange arrows ), CPD and p53 ( green arrows ) or CPD and p53-a382 ( blue arrows ). Broken lines denote the epidermal/dermal border. All images are at 400X magnification

Article Snippet: The membrane was probed with rabbit monoclonal p53 antibody or p53 acetyl K382 and detected using Clarity Western ECL Blotting Substrate detection kit (Bio-Rad) as per the manufacturer’s instructions.

Techniques: Immunohistochemical staining, Staining, Ex Vivo

Journal: BMC Dermatology

Article Title: SIRT1 activation mediates heat-induced survival of UVB damaged Keratinocytes

doi: 10.1186/s12895-017-0060-y

Figure Lengend Snippet: Effect of UVB and/or heat exposure in keratinocytes of the ex vivo skin models or NHEK in vitro

Article Snippet: The membrane was probed with rabbit monoclonal p53 antibody or p53 acetyl K382 and detected using Clarity Western ECL Blotting Substrate detection kit (Bio-Rad) as per the manufacturer’s instructions.

Techniques: Ex Vivo

Journal: BMC Dermatology

Article Title: SIRT1 activation mediates heat-induced survival of UVB damaged Keratinocytes

doi: 10.1186/s12895-017-0060-y

Figure Lengend Snippet: Effect of UV and/or heat exposure on p53 downstream gene targets and cell proliferation. a-b Fold change on mRNA expression of BAX, Survivin, ERCC1 or XPC in keratinocytes of the ( a ) skin and ( b ) in vitro, relative to untreated controls. c-d Bar graphs of the percentage mean (+/− SD) of keratinocytes positive for ki67 per field of view either ( c ) in ex vivo skin or ( d ) in vitro. Statistically significant differences are indicated with ** p < 0.001 and/or *** for p -values p < 0.0001

Article Snippet: The membrane was probed with rabbit monoclonal p53 antibody or p53 acetyl K382 and detected using Clarity Western ECL Blotting Substrate detection kit (Bio-Rad) as per the manufacturer’s instructions.

Techniques: Expressing, In Vitro, Ex Vivo

Journal: BMC Dermatology

Article Title: SIRT1 activation mediates heat-induced survival of UVB damaged Keratinocytes

doi: 10.1186/s12895-017-0060-y

Figure Lengend Snippet: Inhibition of SIRT1 in UVB plus heat significantly increased acetylated p53 protein expression and apoptosis of keratinocytes in vitro. a Immunoblot showing levels of total p53, and acetylated p53 protein in UVB, UVB plus heat and UVB plus heat with the SIRT1 inhibitor (Ex-527). b Quantification of protein levels by relative average density standardised by β-actin. c Representative immunohistochemical staining of cells positive for CPD and p53 ( white arrows ), CPD and p53-a382 ( green arrows ) in primary keratinocytes exposed to UVB or UVB plus heat with or without SIRT1 inhibitor Ex-527. All images are at 400X magnification. d Bar graphs of percentage mean (+/− SD) of DNA damaged (CPD), apoptotic, p53 or p53-a382 positive primary keratinocytes exposed to UVB or UVB plus heat with or without Ex-527. Statistically significant differences are indicated with ** for p < 0.001 and/or *** for p -values p < 0.0001 respectively

Article Snippet: The membrane was probed with rabbit monoclonal p53 antibody or p53 acetyl K382 and detected using Clarity Western ECL Blotting Substrate detection kit (Bio-Rad) as per the manufacturer’s instructions.

Techniques: Inhibition, Expressing, In Vitro, Western Blot, Immunohistochemical staining, Staining